When Bob Loblaw, (played by Scott Baio) says it's not the first time he's had to replace Barry Zuckerkorn as a younger person and "heartthrob", it may be a reference to Baio's character Chachi on Happy Days (1974), appearing on that show as the younger "heartthrob" nephew of Fonzie (played by Henry Winkler).
When Rita (Charlize Theron) is sitting on the "Wee Britain" bus bench she blocks the letters I and T so the sign appears to say "Wee Brain."
In her role as a movie executive, Maeby (Alia Shawkat) green-lights a script by "Judd Apacow", which she re-titles "The 14-Year-Old Virgin". Judd Apatow co-wrote and directed The 40-Year-Old Virgin (2005).
George Bluth (Jeffrey Tambor) gets the idea to escape on a deck chair tied to balloons after watching a scene from the movie Danny Deckchair (2003).
The seal on Rita (Charlize Theron)'s back had a yellow bow tie, the same colour tie as the one that bit off Buster (Tony Hale)'s hand.