Sam says to Dean, "Dogs and cats living together... mass hysteria!". This is a reference to a Peter Venkman line in the movie Ghostbusters (1984): "Human sacrifice, dogs and cats living together... mass hysteria!"
In this episode, the Winchesters go by the aliases "Holt" and "Wilson." In the movie Wolfen (1981), Officers Holt and Wilson investigate killings done by werewolves.
Sam tells Dean that he, Sam, would double cross them, to which Dean replies, "Thanks, Dexter. That's reassuring." Dean is referring to the soulless serial killer title character in the TV crime thriller Dexter (2006).
Lucky is played by actor Andrew Rothenberg, who is probably best known as portraying Jim in season one of The Walking Dead (2010). Series star Jensen Ackles is a huge fan of both the Walking Dead comics and TV series.