At the suspect's house, the blueprints list the blood bank address as 2201 Oriole Lane. However, when Shawn, Gus, and Jules drive up later that night the address listed on the sign attached to the building is listed as 221 Oriole Lane.
When Lassiter holds the necklace at the crime scene, some of chain is caught between his fingers in a couple shots, but not in most others. In the final shot the way he holds it changes completely.
(at around 26 mins) Carlton is asked if he's wearing garters, the camera pans down and they are tautly connected to both his shirt and socks. When Carlton turns and walks away they are no longer attached to his socks, dangling freely from his shirt.
Chloroform doesn't knock someone out instantly like it did to Gus. In reality, chloroform actually takes a while to effectively produce results.
The doctor misspeaks when he says there are only about 24 people in Santa Barbara who have O-negative blood (with a population of about 88,410, there should be about 6,600 people in Santa Barbara with O-negative blood). What he must have meant to say is that there are only about 24 blood donors with O-negative blood in the system.