During the opening fight scene, Batroc the Leaper berates Spider-Man for not knowing that French fries are a Belgian invention and then hops away while singing a snatch of music from the opera "Rigoletto" - which is by an Italian composer.
At one point, the news crawl on Jameson's screen mentions Hingle McCringleberry, a football character from the sketch show Key and Peele (2012)
In this episode, Stan the Janitor stands on a box labeled Stan's Soapbox. Stan's Soapbox was a monthly column written by Stan himself in Marvel Comics.