In the first episode of LOL, one at a time the 10 comedian contestants enter the theater; they don't know who the other contestants are: Frank Matano, Angelo Pintus, Katia Follesa, Lillo, Michela Giraud, Ciro and Fru from The Jackal, Luca Ravenna, Caterina Guzzanti, Elio. Fedez, as the MC and referee, explains the rules of the game to the contestants: they'll have to stay shut in for six hours trying never to laugh, and in the meantime trying to make the others laugh as much as possible. With the help of his co-host Mara Maionchi, Fedez will follow the contest stages from the control room where he'll watch the game on multi-screens and will report if a contestant laughed. The first time someone laughs the yellow card will get pulled as a warning, the second time the red card, eliminating from the game. Whoever makes it to the end without laughing will win EUR100,000 to turn over to charity. Fedez, once done explaining, meets Mara in the control room and kicks off the game. The opponents start "striking each other" with jokes and sketches but keeping a straight face gets harder than some might have thought.
—Endemol Shine Italy